ARCA Cy5 EGFP mRNA (5-moUTP) can be used to analyze mRNA delivery and translation efficiency Upon transfection of cells with ARCA Cy5 EGFP mRNA (5-moUTP) EGFP (Enhanced Green Fluorescent Protein) is expressed EGFP is a frequently used reporter protein that emits strong and bright green fluorescence upon excitation with an excitation/emission maximum of 488 nm/509 nm respectively This mRNA is labeled with the fluorescent dye Cyanine 5 (Cy5) allowing direct visualization of EGFP mRNA upon excitation at specific wavelengths the excitation and emission maxima for Cy5 are 650 nm and 670 nm respectively Cy5 EGFP mRNA is thus ideally suited for analyzing mRNA delivery and localization independently from translation This product is a synthetic mRNA modified by adding a cap structure at the 5 end (Cap) a poly(A) tail at the 3 end and inclusion of the modified nucleotides 5-moUTP and Cyanine 5-UTP The incorporation ratio of Cyanine 5-UTP to 5-moUTP is 1 3 The cap st